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pcag pbase vector  (Addgene inc)


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    Addgene inc pcag pbase vector
    Pcag Pbase Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcag pbase vector/product/Addgene inc
    Average 93 stars, based on 8 article reviews
    pcag pbase vector - by Bioz Stars, 2026-04
    93/100 stars

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    Role of Syk-Cys in phagocytosis. A , the ΔSyk Raw cell line does not express Syk. Western blot of cells following Syk or Firefly luciferase knockout in RAW cells using CRISPR/Cas9. B , Phagocytosis efficiency of Cys to Ser mutants of mSyk following transfection of EGFP-mSyk in ΔSyk RAW cells. Cells were allowed to phagocytose IgG-opsonized 3µm-diameter latex beads for 15 min at 37°C, before counting beads inside and outside cells. See Figure S1 for details.

    Journal: bioRxiv

    Article Title: Syk activation during FcγR-mediated phagocytosis involves both Syk palmitoylation and desulfenylation

    doi: 10.1101/2025.05.26.656092

    Figure Lengend Snippet: Role of Syk-Cys in phagocytosis. A , the ΔSyk Raw cell line does not express Syk. Western blot of cells following Syk or Firefly luciferase knockout in RAW cells using CRISPR/Cas9. B , Phagocytosis efficiency of Cys to Ser mutants of mSyk following transfection of EGFP-mSyk in ΔSyk RAW cells. Cells were allowed to phagocytose IgG-opsonized 3µm-diameter latex beads for 15 min at 37°C, before counting beads inside and outside cells. See Figure S1 for details.

    Article Snippet: To prepare ΔSyk cells we used the Addgene all-in-one CRISPR/Cas9 vector (#79144) as described ( Giuliano et al , 2019 ).

    Techniques: Western Blot, Luciferase, Knock-Out, CRISPR, Transfection