Journal: Proceedings of the National Academy of Sciences of the United States of America
Article Title: Brain-wide mapping of developmental trajectories of cerebellar efferent projections
doi: 10.1073/pnas.2521091122
Figure Lengend Snippet: Development of eCb–hindbrain projections . 3D visualization of the development of excitatory cerebellar (eCb; Ntsr1 Cre +AAV-FLEX-EGFP ) efferent projections innervating the hindbrain. The movie highlights the progressive expansion of eCb axonal pathways across hindbrain regions.
Article Snippet: To anterogradely trace cerebellar long-range projections, we utilized an EGFP lentivirus (LV-H1p-UbCpEGFP) in wild-type mice and Cre-dependent viral vectors pAAV CAG-FLEX-EGFP-WPRE (Addgene #51502, AAV1) or pAAV hSyn.FLEX.mGFP.2A.Synaptophysin-mRuby (Addgene #71760, AAV1) in Ntsr1 Cre and Vgat1 Cre mice.
Techniques: